Detection of human cytomegalovirus in dental plaque from individual periodontal sites by real-time polymerase chain reaction

作者：Combs Darrin R; Reilly Elizabeth A; Dawson Dolphus R III; Avdiushko Sergei A; Danaher Robert J; Miller Craig S^*

来源：Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 2008, 106(6): 840-844.

DOI：10.1016/j.tripleo.2008.06.012

Objective. The aim was to evaluate three primer-probe sets and real-time polymerase chain reaction (PCR) for the detection of human cytomegalovirus (HCMV) in dental plaque from individual periodontal sites.
Study design. Fifty subgingival plaque specimens from 13 healthy subjects (on average at least 2 healthy and 2 periodontal disease sites per subject) and 50 saliva specimens from 24 subjects, including 16 controls, were assessed using 3 primer-probe sets (polymerase [POL], glycoprotein B [gB], and US14) and real-time PCR. Kappa statistics were performed to measure agreement between the primer-probe sets.
Results. There was excellent agreement between the gB and POL primers in the detection of HCMV (kappa statistic = 0.85 [95% confidence interval 0.71-0.99]), yielding a prevalence of 4% (2 out of 50) at individual periodontal disease sites and a similar rate of 8.8% (3 out of 34) in saliva.
Conclusion. Human cytomegalovirus was infrequently detected in dental plaque. Of 3 primer-probe sets evaluated, those targeting the POL and gB genes were more accurate in the detection of HCMV than that targeting US14. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008; 106: 840-844)

• 出版日期2008-12