Non-invasive single-cell biomechanical analysis using live-imaging datasets

作者：Pearson Yanthe E; Lund Amanda W; Lin Alex W H; Ng Chee P; Alsuwaidi Aysha; Azzeh Sara; Gater Deborah L; Teo Jeremy C M

来源：Journal of Cell Science, 2016, 129(17): 3351-3364.

DOI：10.1242/jcs.191205

• Cell biomechanics
• Live imaging
• Mechanobiology
• T-lymphocyte cells
• Mesenchymal stem cells
• Cell migration

The physiological state of a cell is governed by a multitude of processes and can be described by a combination of mechanical, spatial and temporal properties. Quantifying cell dynamics at multiple scales is essential for comprehensive studies of cellular function, and remains a challenge for traditional end-point assays. We introduce an efficient, non-invasive computational tool that takes time-lapse images as input to automatically detect, segment and analyze unlabeled live cells; the program then outputs kinematic cellular shape and migration parameters, while simultaneously measuring cellular stiffness and viscosity. We demonstrate the capabilities of the program by testing it on human mesenchymal stem cells (huMSCs) induced to differentiate towards the osteoblastic (huOB) lineage, and T-lymphocyte cells (T cells) of naive and stimulated phenotypes. The program detected relative cellular stiffness differences in huMSCs and huOBs that were comparable to those obtained with studies that utilize atomic force microscopy; it further distinguished naive from stimulated T cells, based on characteristics necessary to invoke an immune response. In summary, we introduce an integrated tool to decipher spatiotemporal and intracellular dynamics of cells, providing a new and alternative approach for cell characterization.

• 出版日期2016-9-1