Stem potentialities of the human iris - An in situ immunohistochemical study

作者：Vrapciu Alexandra Diana; Rusu Mugurel Constantin^*; Leonardi Rosalia; Corbu Catalina Gabriela

来源：Acta Histochemica, 2014, 116(8): 1509-1513.

DOI：10.1016/j.acthis.2014.07.013

• Stem cells
• Progenitor cells
• Circulating fibrocytes
• Fibroblasts
• Angiogenesis
• Iris

According to recent findings multiple human tissues harbor stem cells which, in turn, have different levels of stemness. We performed an immunohistochemical study on paraffin-embedded samples to test if the in situ stromal cells of the iris of the human eye (EI) have immune stem/progenitor phenotypes. Eviscerated post-traumatic eyes from eight patients were studied. These irises were found to contain fibroblastoid stromal cells with a CD34+/CD45+/CD105+/CD117+/DOG1+/PDGFR-alpha+/vimentin+/nestin-/collagen III- phenotype. These were assumed to be possible stem/progenitor cells involved in physiological processes of iridial stromal maintenance. All the vascular endothelia were CD34+/CD105+/vimentin+. Newly formed nestin+endothelia were also found; this finding was supported by evidence of filopodia-projecting CD34+ endothelial tip cells, which demonstrated active processes of sprouting angiogenesis. The phenotype of the stromal cells also suggests a role of the circulating fibrocytes in iridial regenerative processes.

• 出版日期2014