No training required: experimental tests support homology-based DNA assembly as a best practice in synthetic biology

作者：Azizi Afnan; Lam Wilson; Phenix Hilary; Tepliakova Lioudmila; Roney Ian J; Jedrysiak Daniel; Power Alex; Gupta Vaibhav; Elnour Nada; Hanzel Martin; Tzahristos Alexandra C; Sarwar Shihab; Kaern Mads^*

来源：Journal of Biological Engineering, 2015, 9(1): 8.

DOI：10.1186/s13036-015-0006-z

• Synthetic biology
• DNA assembly
• Cloning
• Gibson
• Seamless
• iGEM
• PCR

The Registry of Standard Biological Parts imposes sequence constraints to enable DNA assembly using restriction enzymes. Alnahhas et al. (Journal of Biological Engineering 2014, 8:28) recently argued that these constraints should be revised because they impose an unnecessary burden on contributors that use homology-based assembly. To add to this debate, we tested four different homology-based methods, and found that students using these methods on their first attempt have a high probability of success. Because of their ease of use and high success rates, we believe that homology-based assembly is a best practice of Synthetic Biology, and recommend that the Registry implement the changes proposed by Alnahhas et al. to better support their use.

• 出版日期2015-6-12