Modulation of the multidrug efflux pump EmrD-3 from Vibrio cholerae by Allium sativum extract and the bioactive agent allyl sulfide plus synergistic enhancement of antimicrobial susceptibility by A-sativum extract

作者：Bruns Merissa M; Kakarla Prathusha; Floyd Jared T; Mukherjee Mun Mun; Ponce Robert C; Garcia John A; Ranaweera Indrika; Sanford Leslie M; Hernandez Alberto J; Willmon T Mark; Tolson Grace L; Varela Manuel F^*

来源：Archives of Microbiology, 2017, 199(8): 1103-1112.

DOI：10.1007/s00203-017-1378-x

• Multidrug efflux pump
• Antimicrobial resistance
• Bacteria
• Garlic
• Medicinal plants
• Vibrio cholerae
• Synergy
• Allyl sulfide
• Allium sativum

The causative agent of cholera, Vibrio cholerae, is a public health concern. Multidrug-resistant V. cholerae variants may reduce chemotherapeutic efficacies of severe cholera. We previously reported that the multidrug efflux pump EmrD-3 from V. cholerae confers resistance to multiple structurally distinct antimicrobials. Medicinal plant compounds are potential candidates for EmrD-3 efflux pump modulation. The antibacterial activities of garlic Allium sativum, although poorly understood, predicts that a main bioactive component, allyl sulfide, modulates EmrD-3 efflux. Thus, we tested whether A. sativum extract acts in synergy with antimicrobials and that a main bioactive component allyl sulfide inhibits EmrD-3 efflux. We found that A. sativum extract and allyl sulfide inhibited ethidium bromide efflux in cells harboring EmrD-3 and that A. sativum lowered the MICs of multiple antibacterials. We conclude that A. sativum and allyl sulfide inhibit EmrD-3 and that A. sativum extract synergistically enhances antibacterial agents.

• 出版日期2017-10