A fraction of methylene chloride from Geum japonicum Thunberg inhibits tumor metastatic and angiogenic potential

作者：Heo Jin Chul; Son Minsik; Woo Sang Uk; Kweon Mi Ae; Yoon Eun Kyung; Lee Hee Kyung; Choi Won Sik; Cho Kang Jin; Lee Sang Han^*

来源：Oncology Reports, 2008, 19(6): 1399-1403.

• Geum japonicum Thunberg
• angiogenesis
• cell migration
• mitogen-activated protein kinase

The plant Geum japonicum Thunberg (GjT) has been used as a diuretic in traditional medicine. Herein, we report that the GjT extract blocks both the spread of human umbilical vein endothelial cells (HUVECs) on matrigel and the migration of B 16 cells. We used various assays to test for cell attachment, spreading, wound healing and angiogenesis. A reverse transcription-polymerase chain reaction (RT-PCR) and a mitogen-activated protein kinase (MAPK) assay were also carried out for the mechanistic study of GjT. Our results showed that a fraction of methylene chloride fraction from G(j)T inhibited B16 cells during cell attachment and migration and suppressed tube formation in a dose-dependent manner. An RT-PCR analysis showed that the methylene chloride extract decreased the mRNA expression of CD44 and TIMP-2. A Western blot analysis of the phosphorylation of MAPK kinases (ERK, JNK and p38) showed that the GJT fraction increased the expression of phospho-JNK, suggesting that GjT has. the potential to alleviate metastatic and angiogenic activity, via a phospho-JNK signaling pathway.

• 出版日期2008-6