An investigation of endocrine disrupting effects and toxic mechanisms modulated by benzo[a]pyrene in female scallop Chlamys farreri

作者：Tian, Shuangmei; Pan, Lucting^*; Sun, Xiaohua

来源：Aquatic Toxicology, 2013, 144: 162-171.

DOI：10.1016/j.aquatox.2013.09.031

• Benzo[a]pyrene
• Chlamys farreri
• Endocrine disruption
• Steroidogenesis
• AHR
• ER

The purpose of this study was to investigate the endocrine disrupting effects induced by benzo[a]pyrene (B[a]P) and explore the underlying mechanisms in mollusks. In this study, sexually mature female Chlamys farreri were exposed to benzo[a]pyrene for 10 days at four different concentrations as 0, 0.025, 0.5 and 10 mu g/L. Sex steroids were identified and quantified by electrochemiluminescence immunoassay (ECLIA) method and results showed that exposure to B[a]P exerts great suppression on 17 beta-estradiol, testosterone production and disrupts progesterone levels in ovary. Transcription of genes were detected and measured by real-time RT-PCR. It showed that at day 10 B[a]P inhibited 3 beta-HSD, CYP17 and 17 beta-HSD mRNA expression in a dose-dependent manner, which suggests that they could be potential targets of B[a]P that disrupt steroidogenic machinery. Moreover, 0.025 mu g/L B[a]P activated transcription of aryl hydrocarbon receptor (AHR), AHR nuclear translocator (ARNT), CYP1A1 and estrogen receptor (ER), while 10 mu g/L B[a]P suppressed all of them. The consistency of their responses to B[a]P exposure implies that AHR action may be involved in invertebrate CYP regulation and ER transcription despite of unknown mechanisms. Additionally, B[a]P exposure could induce ovarian impairment and developmental delay in C farreri. Overall, sensitivity of C. farreri to endocrine disruption and toxicity suggests that C farreri is a suitable species for study of endocrine-disrupting effects in marine invertebrates. This study will form a solid basis for a realistic extrapolation of endocrine disrupting effects across taxonomic groups and phyla.

• 出版日期2013-11-15
• 单位中国海洋大学