Antioxidant capacity and cytotoxicity of sulfated polysaccharide TLH-3 from Tricholoma lobayense

作者：Li, Xuehui; Lu, Yongming; Zhang, Wenna; Yuan, Shixiong; Zhou, Liyuan; Wang, Liming; Ding, Qiuying; Wang, Dandan; Yang, Weiwei; Cai, Zhengnan; Chen, Yan^*

来源：International Journal of Biological Macromolecules, 2016, 82: 913-919.

DOI：10.1016/j.ijbiomac.2015.10.006

• Tricholoma lobayense sulfated polysaccharide
• Antioxidant
• Cytotoxicity

Polysaccharide TLH-3 from the fruit body of Tricholoma lobayense Heim has shown outstanding antioxidant activity. In order to further explore it,TLH-3 was successfully modified to obtain a sulfated derivative (STLH-3). The chemical characteristics of STLH-3 and TLH-3 were determined by high performance liquid chromatography and infrared spectroscopy. Antioxidant activity and cytotoxicity of the samples were investigated in vitro. The antioxidant activities of STLH-3 were significantly improved. In the four indicators of antioxidant activity, the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion scavenging activities of STLH-3 have exceeded that of Vitamin C (Vc). The cytotoxicity against tumor cells including human cervical carcinoma cell line (HeLa) and human breast cancer cell line (MCF-7) of STLH-3 was obviously stronger than that of TLH-3. STLH-3 had no toxic on human embryonic lung fibroblasts (HELF) cells. These results suggested that STLH-3 would be a promising bioactive macromolecule for potential applications in the field of health care and pharmaceutical.

• 出版日期2016-1
• 单位安徽大学