Direct electron transfer of glucose oxidase and biosensing for glucose based on PDDA-capped gold nanoparticle modified graphene/multi-walled carbon nanotubes electrode

作者：Yu, Yanyan; Chen, Zuanguang^*; He, Sijing; Zhang, Beibei; Li, Xinchun; Yao, Meicun

来源：Biosensors and Bioelectronics, 2014, 52: 147-152.

DOI：10.1016/j.bios.2013.08.043

• Direct electron transfer
• Gold nanoparticle
• Multi-walled carbon nanotube
• Graphene
• Glucose oxidase
• Biosensor

In this work, poly (diallyldimethylammonium chloride) (PDDA)-capped gold nanoparticles (AuNPs) functionalized graphene (G)/multi-walled carbon nanotubes (MWCNTs) nanocomposites were fabricated. Based on the electrostatic attraction, the G/MWCNTs hybrid material can be decorated with AuNPs uniformly and densely. The new hierarchical nanostructure can provide a larger surface area and a more favorable microenvironment for electron transfer. The AuNPs/G/MWCNTs nanocomposite was used as a novel immobilization platform for glucose oxidase (GOD). Direct electron transfer (DET) was achieved between GOD and the electrode. Field emission scanning electron microscopy (FESEM), UV-vis spectroscopy and cyclic voltammetry (CV) were used to characterize the electrochemical biosensor. The glucose biosensor fabricated based on GOD electrode modified with AuNPs/G/MWCNTs demonstrated satisfactory analytical performance with high sensitivity (29.72 mA M-1 cm(-2)) and low limit of detection (4.8 mu M). The heterogeneous electron transfer rate constant (K-s) and the apparent Michaelis-Menten constant (K-m) of GOD were calculated to be 11.18 s(-1) and 2.09 mM, respectively. With satisfactory selectivity, reproducibility, and stability, the nanostructure we proposed offered an alternative for electrode fabricating and glucose biosensing.

• 出版日期2014-2-15
• 单位中山大学; 广西医科大学