Antispasmodic effect of Mentha piperita essential oil on tracheal smooth muscle of rats

作者：Sydney de Sousa Albertina Antonielly; Gomes Soares Pedro Marcos; Saldanha de Almeida Arisa Nara; Maia Alana Rufino; de Souza Emmanuel Prata; Sampaio Assreuy Ana Maria

来源：Journal of Ethnopharmacology, 2010, 130(2): 433-436.

DOI：10.1016/j.jep.2010.05.012

• Essential oil
• Mentha piperita
• Trachea
• Smooth muscle
• Antispasmodic activity

Aim of the study: Mentha piperita is a plant popularly known in Brazil as "hortela-pimenta" whose essential oil is used in folk medicine for its anti-inflammatory, antispasmodic, expectorant actions and anti-congestive. Here, it was investigated the effect of Mentha piperita essential oil (peppermint oil) in rat tracheal rings along with its mechanism of action.
Materials and methods: Tracheal tissue from Male Wistar rats (250-300g) were used. Peppermint oil was added in cumulative concentrations [1-300 mu g/ml] to the tissue basal tonus or pre-contracted by carbachol [10 mu M] at 10 min intervals, incubated or not with indomethacin [10 mu M], t-N-metyl-nitro-arginine [100 mu M], hexamethonium 1500 mu M], or tetraethylammonium [5mM].
Results: Peppermint oil [100 and 300 mu g/ml] inhibited the contractions induced by carbachol, which was reversed by indomethacin, t-N-metyl-nitro-arginine and hexamethonium, but not by tetraethylammonium. These data suggest the participation of prostaglandin E(2), nitric oxide and autonomic ganglions in the peppermint oil relaxant effect and may be correlated with its popular use in respiratory diseases.
Conclusions: Peppermint oil exhibited antispasmodic activity on rat trachea involving prostaglandins and nitric oxide synthase.

• 出版日期2010-7-20