摘要

The analysis and separation of complex samples by multidimensional liquid column chromatography are paid much attention. In this paper, the recent progress of multidimensional liquid column chromatography is reviewed. The construction of two dimensional liquid chromatography is discussed in detail, including the selections of stationary phases, mobile phases, the function of temperature variable and the switching between the two dimensions. The applications of multidimensional liquid column chromatography are summarized.