A study on the short-term effect of cafeteria diet and pioglitazone on insulin resistance and serum levels of adiponectin and ghrelin

作者：Colombo G^*; Bazzo M L; Nogueira C L; Colombo M D H P; Schiavon L L; d'Acampora A J

来源：Brazilian Journal of Medical and Biological Research, 2012, 45(10): 935-941.

DOI：10.1590/S0100-879X2012007500117

• Diet
• Insulin resistance
• Adiponectin
• Ghrelin
• Thiazolidinediones

The interaction between ghrelin and adiponectin is still controversial. We investigated the effect of cafeteria diet and pioglitazone on body weight, insulin resistance, and adiponectin/ghrelin levels in an experimental study on male Wistar rats. The animals were divided into four groups of 6 rats each, and received balanced chow with saline (CHOW-O) or pioglitazone (CHOW-P), or a cafeteria diet with saline (CAFE-O) or pioglitazone (CAFE-P). The chow/cafeteria diets were administered for 35 days, and saline/pioglitazone (10 mg.kg body weight(-1).day(-1)) was added in the last 14 days prior to euthanasia. CAFE-O animals had a higher mean final weight (372.5 +/- 21.01 g) than CHOW-O (317.66 +/- 25.11 g, P = 0.017) and CHOW-P (322.66 +/- 28.42 g, P = 0.035) animals. Serum adiponectin levels were significantly higher in CHOW-P (55.91 +/- 20.62 ng/mL) than in CHOW-O (30.52 +/- 6.97 ng/mL, P = 0.014) and CAFE-O (32.54 +/- 9.03 ng/mL, P = 0.027) but not in CAFE-P. Higher total serum ghrelin levels were observed in CAFE-P compared to CHOW-P animals (1.65 +/- 0.69 vs 0.65 +/- 0.36 ng/mL, P = 0.006). Likewise, acylated ghrelin levels were higher in CAFE-P (471.52 +/- 195.09 pg/mL) than in CHOW-P (193.01 +/- 87.61 pg/mL, P = 0.009) and CAFE-O (259.44 +/- 86.36 pg/mL, P = 0.047) animals. In conclusion, a cafeteria diet can lead to a significant weight gain. Although CAFE-P animals exhibited higher ghrelin levels, this was probably related to food deprivation rather than to a direct pharmacological effect, possibly attenuating the increase in adiponectin levels.

• 出版日期2012-10