Generation of novel high quality HMW-GS genes in two introgression lines of Triticum aestivum/Agropyron elongatum

作者：Liu Shuwei; Zhao Shuangyi; Chen Fanguo; Xia Guangmin^*

来源：BMC Evolutionary Biology, 2007, 7: 76.

DOI：10.1186/1471-2148-7-76

Background: High molecular weight glutenin subunits ( HMW-GS) have been proved to be mostly correlated with the processing quality of common wheat ( Triticum aestivum). But wheat cultivars have limited number of high quality HMW-GS. However, novel HMW-GS were found to be present in many wheat asymmetric somatic hybrid introgression lines of common wheat/Agropyron elongatum.
Results: To exploit how these new subunits were generated, we isolated HMW-GS genes from two sib hybrid lines ( II-12 and II-4-6) and compared them with those from their parents. The result shows that two genes of hybrid ( HII-3-3 and HII-4-3) are directly introgressed from the donor parent Agropyron elongatum; one hybrid gene ( HIDx5) comes from point mutation of a parental wheat gene ( IDx2.1); two other hybrid genes ( HIBy8 and HIBy16) are likely resulting from unequal crossover or slippage of a parental wheat gene ( IBy9.1); and the sixth novel hybrid gene ( HIDy12) may come from recombination between two parental genes.
Conclusion: Therefore, we demonstrate that novel HMW-GS genes can be rapidly created through asymmetric somatic hybridization in a manner similar with the evolution mechanism of these genes supposed before. We also described gene shuffling as a new mechanism of novel HMW-GS gene formation in hybrids. The results suggest that asymmetric somatic hybridization is an important approach for widening HMW-GS genebank of wheat quality improvement.

• 出版日期2007-5-15
• 单位山东大学