MicroRNAs as a potential prognostic factor in gastric cancer

作者：Brenner Baruch^*; Hoshen Moshe B; Purim Ofer; Ben David Miriam; Ashkenazi Karin; Marshak Gideon; Kundel Yulia; Brenner Ronen; Morgenstern Sara; Halpern Marisa; Rosenfeld Nitzan; Chajut Ayelet; Niv Yaron; Kushnir Michal

来源：World Journal of Gastroenterology, 2011, 17(35): 3976-3985.

DOI：10.3748/wjg.v17.i35.3976

• MicroRNA
• Prognosis
• Recurrence
• Gastric cancer

AIM: To compare the microRNA (miR) profiles in the primary tumor of patients with recurrent and non-recurrent gastric cancer.
METHODS: The study group included 45 patients who underwent curative gastrectomies from 1995 to 2005 without adjuvant or neoadjuvant therapy and for whom adequate tumor content was available. Total RNA was extracted from formalin-fixed paraffin-embedded tumor samples, preserving the small RNA fraction. Initial profiling using miR microarrays was performed to identify potential biomarkers of recurrence after resection. The expression of the differential miRs was later verified by quantitative real-time polymerase chain reaction (qRT-PCR). Findings were compared between patients who had a recurrence within 36 mo of surgery (bad-prognosis group, n = 14, 31%) and those who did not (good-prognosis group, n = 31, 69%).
RESULTS: Three miRs, miR-451, miR-199a-3p and miR-195 were found to be differentially expressed in tumors from patients with good prognosis vs patients with bad prognosis (P < 0.0002, 0.0027 and 0.0046 respectively). High expression of each miR was associated with poorer prognosis for both recurrence and survival. Using miR-451, the positive predictive value for non-recurrence was 100% (13/13). The expression of the differential miRs was verified by qRT-PCR, showing high correlation to the microarray data and similar separation into prognosis groups.
CONCLUSION: This study identified three miRs, miR-451, miR-199a-3p and miR-195 to be predictive of recurrence of gastric cancer. Of these, miR-451 had the strongest prognostic impact.

• 出版日期2011-9-21