Nanocalorimetry of respiration in micro-organisms in natural waters

作者：Mukhanov Vladimir S; Hansen Lee D^*; Kemp Richard B

来源：Thermochimica Acta, 2012, 531: 66-69.

DOI：10.1016/j.tca.2012.01.001

• Calorimetry
• Respiration
• Chlorella vulgaris
• Duna liella maritime
• Micrococcus luteus
• Seawater
• Microplankton

Two commercially available nanocalorimeters were assessed for direct measurement of respiration rates of micro-organisms in natural waters or liquid cultures as a function of temperature. Respiration heat rates were measured with isothermal and temperature-scanning nanocalorimeters in natural seawater and in cultures of microplankton and bacteria. The standard deviation of a measurement of metabolic heat rate by isothermal nanocalorimetry is +/- 25 nW/mL and by temperature-scanning nanocalorimetry, +/- 150 nW/mL as a continuous function of temperature. Although the absolute uncertainty of temperature scanning calorimetry is larger, the relative noise between data points is small and temperature scanning rapidly produces a curve of heat rate versus temperature that describes the metabolic response to temperature. Used together, the two methods are capable of generating a curve of metabolic heat rate versus temperature over the entire temperature range of respiratory activity in about 4h.

• 出版日期2012-3-10