Detection of A beta Plaques by a Novel Specific MRI Probe Precursor CR-BSA-(Gd-DTPA)n in APP/PS1 Transgenic Mice

作者：Li, Shuping; He, Huijin; Cui, Weigang; Gu, Bing; Li, Jin; Qi, Zengxin; Zhou, Guomin; Liang, Chun-Min^*; Feng, Xiao-Yuan

来源：Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology, 2010, 293(12): 2136-2143.

DOI：10.1002/ar.21209

• A beta plaques
• MRI probe
• CR-BSA-(Gd-DTPA)n
• APP/PS1
• transgenic mouse

Amyloid plaques are one of the hallmarks of Alzheimer's disease (AD). The lack of specific probes that can detect individual senile plaques in AD has prompted the development of magnetic resonance imaging (MRI) probes. In this study, based on DTPA-gadolinium (III) and congo red (CR), a novel specific MRI probe precursor CR-BSA-(Gd-DTPA)n was successfully synthesized. Its ability to bind to amyloid plaques was evaluated by brain sections from APP/PS1 transgenic mice. Its specificity for A beta plaques was further demonstrated by immunohistochemistry (IHC) staining with the monoclonal antibody to the A beta protein. Meanwhile, the amyloid deposits detected by the CR-BSA-(Gd-DTPA)n were matched to the amyloid deposits detected by A beta specific antibody. We also found that a few amyloid-like deposits which was not detected by IHC. The findings indicated that the probe perhaps could detect the neurofibrillary tangles (NFT) similar to the effect of CR itself, and this will be verified in future experiments. The works suggested that the A beta protein-specific magnetic resonance contrast agent precursor CR-BSA-(Gd-DTPA)n can be used as a potential fluorescence and MR multi-modal imaging probe precursor to display individual senile plaques in AD. Anat Rec, 293:2136-2143,2010.

• 出版日期2010-12
• 单位中国人民解放军第四五五医院; 复旦大学