Normal and polar-organic-phase high-performance liquid chromatographic enantioresolution of omeprazole, rabeprazole, lansoprazole and pantoprazole using monochloro-methylated cellulose-based chiral stationary phase and determination of dexrabeprazole

作者：Dixit Shuchi^*; Dubey Rituraj; Bhushan Ravi

来源：Biomedical Chromatography, 2014, 28(1): 112-119.

DOI：10.1002/bmc.2915

• high-performance liquid chromatography
• enantioresolution
• chiral sulfoxides
• monochloro methylated cellulose as chiral stationary phase
• dexrabeprazole

Enantioresolution of four anti-ulcer drugs (chiral sulfoxides), namely, omeprazole, rabeprazole, lansoprazole and pantoprazole, was carried out by high-performance liquid chromatography using a polysaccharide-based chiral stationary phase consisting of monochloromethylated cellulose (Lux cellulose-2) under normal and polar-organic-phase conditions with ultraviolet detection at 285nm. The method was validated for linearity, accuracy, precision, robustness and limit of detection. The optimized enantioresolution method was compared for both the elution modes. The optimized method was further utilized to check the enantiomeric purity of dexrabeprazole.

• 出版日期2014-1