A critical body residue approach for predicting persistent bioaccumulative toxicant effects on reproduction and population dynamics of meiobenthic copepods

作者：Chandler G Thomas^*; Ferguson P Lee; Klauber W W; Washburn K M

来源：Environmental Toxicology and Chemistry, 2012, 31(5): 1076-1082.

DOI：10.1002/etc.1766

• Agricultural chemicals
• Aquatic toxicology
• Bioaccumulative compounds
• Estuarine toxicology
• Population modeling

Critical body residues (CBRs) are the measured tissue toxicant concentrations yielding a median doseresponse on a dry-weight or lipid-normalized basis. They facilitate management decisions for species protection using tissue analysis. Population CBR is the mean dose yielding 50% population suppression and was predicted here in Amphiascus tenuiremis for fipronil sulfide (FS) using lifetables and the Leslie matrix. Microplate bioassays (ASTM E-2317-14) produced biomass sufficient for dry mass and lipid-normalized CBR estimates of reproduction (fertility) and population growth suppression. Significant FS toxic effects were delayed naupliar development (at =0.10 mu gL-1), delayed copepodite development (at 0.85 mu gL-1), decreased reproductive success (at = 0.39 mu gL-1), and decreased offspring production (at 0.85 mu gL-1). A reproductive median effective concentration (EC50) of 0.16 mu gL-1 (95% CI: 0.120.21 mu gL-1) corresponded to an adult all-sex CBR and lipid-normalized CBR of 0.38pg FS center dot mu g-1 dry weight (95% CI: 0.270.52pg FS center dot mu g-1) or 2.8pg FS center dot mu g-1 lipid (95% CI: 2.23.6pg FS center dot mu g-1), respectively. Copepod log bioconcentration factor (BCF)=4.11 +/- 0.2. Leslie matrix projections regressed against internal dose predicted fewer than five gravid females in a population by the third generation at 0.39 and 0.85 mu g FS center dot L-1 (i.e., 9.610.2 mu g FS center dot mu g-1 lipid), and 50% population suppression at a CBR of 1.6pg FS center dot mu g-1 lipid. This more integrative population CBR as a management tool would fall 1.75 times below the CBR for the single most sensitive endpointfertility rate. Environ. Toxicol. Chem. 2012; 31: 10761082.

• 出版日期2012-5