Analysis of the grapevine moth Lobesia botrana antennal transcriptome and it expression of odorant-binding and chemosensory proteins

作者：Rojas Valentina; Jimenez Hector; Palma Millanao Ruben; Gonzalez Gonzalez Angelica; Machuca Juan; Godoy Ricardo; Ceballos Ricardo; Mutis Ana; Venthur Herbert^*

来源：Comparative Biochemistry and Physiology D-Genomics & Proteomics, 2018, 27: 1-12.

DOI：10.1016/j.cbd.2018.04.003

• Lobesia botrana
• Chemosensory receptors
• Soluble proteins
• Relative expression
• Antennae

The grapevine moth, Lobesia botrana, is considered a harmful pest for vineyards in Chile as well as in North America and Europe. Currently, monitoring and control methods of L. botrana are based on its main sex pheromone component, being effective for low population densities. In order to improve control methods, antennal olfactory proteins in moths, such as odorant-binding proteins (OBPs) and odorant receptors (ORs) have been studied as promising targets for the discovery of new potent semiochemicals, which have not been reported for L. botrana. Therefore, the objective of this study was to identify the repertoire of proteins related to chemoreception in L. botrana by antennal transcriptome and analyze the relative expression of OBPs and CSPs in male and female antennae. Through next-generation sequencing of the antennal transcriptome by Ilumina HiSeq2500 we identified a total of 118 chemoreceptors, from which 61, 42 and 15 transcripts are related to ORs, ionotropic receptors (IRs) and gustatory receptors (GRs), respectively. Furthermore, RNA-Seq data revealed 35 transcripts for OBPs and 18 for chemosensory proteins (CSPs). Analysis by qRT-PCR showed 20 OBPs significantly expressed in female antennae, while 5 were more expressed in males. Similarly, most of the CSPs were significantly expressed in female than male antennae. All the olfactory-related sequences were compared with homologs and their phylogenetic relationships elucidated. Finally, our findings in relation to the improvement of L. botrana management are discussed.

• 出版日期2018-9