A novel mimovirus encoding ChgA(10-19) peptide with PD-L1 induces T cell tolerance and ameliorates the severity of diabetes

作者：Sun, Hong; Han, Xiaoguang; Yan, Xiuhui; Xu, Jingli; Huang, Qiujing; Meng, Fanqing; Zhang, Hongjin; Li, Shufa^*

来源：Cellular Immunology, 2017, 320: 56-61.

DOI：10.1016/j.cellimm.2017.09.001

• Mimovirus
• Chg(A10-19)
• PD-L1
• T cell tolerance
• Diabetes

Related studies demonstrate that type 1 diabetes (T1D) is caused by beta-cell antigen specific autoreactive CD8+ T cells. ChgA has recently been identified as the autoantigen in NOD mice and T1D patients. Therefore, attenuating the activation of ChgA specific CD8+ T cells might be a promising target for T1D therapy. The negative costimulatory PD-L1 inhibits T cell mediated alloimmunity and induces tolerance. In this experiment, a novel mimovirus encoding ChgA10-19 peptide with PD-L1 was constructed. The NOD. beta 2m null HHD mice were administrated with mimovirus transduced DCs. After immunization, the activation and proliferation of CD8+ T cells were detected, diabetes incidence and pancreatic tissue destruction were also analyzed. The results demonstrated that transduced DCs attenuated CD8+ T cell activation and proliferation. In addition, transduced DCs inhibited CD8+ T response to ChgA stimulation, and ameliorated the severity of diabetes. These data suggested that mimovirus transduced DCs might provide novel clues for T1D therapy.

• 出版日期2017-10
• 单位临沂市人民医院