A noninvasive, direct real-time PCR method for sex determination in multiple avian species

作者:Brubaker Jessica L; Karouna Renier Natalie K*; Chen Yu; Jenko Kathryn; Sprague Daniel T; Henry Paula F P
来源:Molecular Ecology Resources, 2011, 11(2): 415-417.
DOI:10.1111/j.1755-0998.2010.02951.x

摘要

Polymerase chain reaction (PCR)-based methods to determine the sex of birds are well established and have seen few modifications since they were first introduced in the 1990s. Although these methods allowed for sex determination in species that were previously difficult to analyse, they were not conducive to high-throughput analysis because of the laboriousness of DNA extraction and gel electrophoresis. We developed a high-throughput real-time PCR-based method for analysis of sex in birds, which uses noninvasive sample collection and avoids DNA extraction and gel electrophoresis.

  • 出版日期2011-3
  • 单位Virginia Tech; 美国弗吉尼亚理工大学(Virginia Tech)