Assessing remyelination - metabolic labeling of myelin in an animal model of multiple sclerosis

作者：Aharoni Rina^*; Rosen Chava; Shezen Elias; Bar Lev Dekel D; Golani Ofra; Reisner Yair; Sela Michael; Arnon Ruth

来源：Journal of Neuroimmunology, 2016, 301: 7-11.

DOI：10.1016/j.jneuroim.2016.10.015

• Multiple sclerosis (MS)
• Experimental autoimmune encephalomyelitis (EAE)
• Remyelination
• In vivo metabolic labeling
• Quantitative colocalization analysis
• Glatiramer acetate (GA)

The lack of biomarkers is a major obstacle for investigating myelin repair. We used metabolic incorporation of the choline analog - propargyl-choline (P-Cho) to label and visualize newly synthesized myelin in the CNS of mice induced with experimental autoimmune encephalomyelitis (EAE). We further developed unbiased colocalization analysis to quantify P-Cho incorporation specifically into the myelin. Our findings indicate that P-Cho injection to mice recovering from EAE, either spontaneously or following glatiramer acetate treatment, results in significant elevation of its incorporation into the myelin, offering a novel strategy for assessing remyelination in animal models and the remyelination potential of candidate drugs.

• 出版日期2016-12-15