Diallyl sulfide, diallyl disulfide and diallyl trisulfide affect drug resistant gene expression in colo 205 human colon cancer cells in vitro and in vivo

作者：Lai Kuang Chi; Kuo Chao Lin; Ho Heng Chien; Yang Jai Sing; Ma Chia Yu; Lu Hsu Feng; Huang Hui Ying; Chueh Fu Shin; Yu Chien Chih^*; Chung Jing Gung

来源：Phytomedicine, 2012, 19(7): 625-630.

DOI：10.1016/j.phymed.2012.02.004

• Garlic
• Diallyl polysulfides
• Drug resistant gene
• Colo 205 human colon cancer cells

To elevate chemo-resistance of human cancer cells is a major obstacle in the treatment and management of malignant cancers. Diallyl sulfide (DAS), diallyl disulfide (DADS) and diallyl trisulfide (DATS) are presented in the Alliaceae family particularly in garlic. Although DAS, DADS and DATS have been shown to exhibit anticancer activities, there is little information on effects of these compounds on drug resistant genes in human colon cancer cells in vitro and in vivo. Herein, we are the first to show that DAS, DADS and DATS at 25 mu M for 24-h and 48-h incubations promoted expression of drug resistant genes in colo 205 human colon cancer cells. In vitro experiments indicated that DATS promoted gene expression of multidrug resistant 1 (Mdr1)(p %26lt; 0.05), and DAS and DADS promoted MRP3 gene expression and DATS alone stimulated gene expression of multidrug resistance-associated protein-1 (MRP1)(p %26lt; 0.05) in colo 205 cells. In vivo studies demonstrated that DADS and OATS induced Mdr1 and MRP1 gene expression (p %26lt; 0.05). DADS promoted MRP3 gene expression (p %26lt; 0.05) as well as DADS and DATS increased MRP4 and MRP6 gene expression (p %26lt; 0.05) in the cob o 205 xenograft mice. Based on our in vitro and in vivo results, diallyl polysulfides (DAS, DADS and DATS) affected the gene expression of the multidrug resistance in cob o 205 human colon cancer cells in vitro and in vivo.

• 出版日期2012-5-15
• 单位中国医科大学