A novel flow injection spectrophotometric method using plant extracts as green reagent for the determination of doxycycline

作者：Palamy Sysay; Ruengsitagoon Wirat^*

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2017, 171: 200-206.

DOI：10.1016/j.saa.2016.08.011

• Doxycycline
• Green reagent
• Flow injection analysis
• Iron(III)

A novel flow injection spectrophotometric method was developed for the determination of doxycycline in pharmaceutical preparations using iron(III) contained in extracts from plants. The assay was based on the complex formed between doxycycline and iron(III) characterized by an absorption maximum at 435 nm. The calibration graphs obtained over the doxycycline concentration range 5-250 mu g mL(-1) gave correlation coefficients of 0.9979, 0.9987 and 0.9987 with the three green reagents prepared from Senna alata (L.) Roxb. (S. alata), Polygonum hydropiper L. (P. hydropiper) or Diplazium esculentum (Betz.) Sw. (D. esculentum), respectively. The relative standard deviations of the repeatability was <2.00%. The percentage recoveries were in the range of 98.27-101.03%. Doxycycline contents obtained by this new method and by the reference methods reported in literature were in agreement at 95% confidence level with the paired t-test. The sample throughput was 36 h(-1) for each green reagent.

• 出版日期2017-1-15