A Hybrid Drug Limits Resistance by Evading the Action of the Multiple Antibiotic Resistance Pathway

作者：Wang Kathy K; Stone Laura K; Lieberman Tami D; Shavit Michal; Baasov Timor; Kishony Roy^*

来源：Molecular Biology and Evolution, 2016, 33(2): 492-500.

DOI：10.1093/molbev/msv243

• antibiotic resistance
• experimental evolution
• hybrid drug
• multidrug resistance

Hybrid drugs are a promising strategy to address the growing problem of drug resistance, but the mechanism by which they modulate the evolution of resistance is poorly understood. Integrating high-throughput resistance measurements and genomic sequencing, we compared Escherichia coli populations evolved in a hybrid antibiotic that links ciprofloxacin and neomycin B with populations evolved in combinations of the component drugs. We find that populations evolved in the hybrid gain less resistance than those evolved in an equimolar mixture of the hybrid's components, in part because the hybrid evades resistance mediated by the multiple antibiotic resistance (mar) operon. Furthermore, we find that the ciprofloxacin moiety of the hybrid inhibits bacterial growth whereas the neomycin B moiety diminishes the effectiveness of mar activation. More generally, comparing the phenotypic and genotypic paths to resistance across different drug treatments can pinpoint unique properties of new compounds that limit the emergence of resistance.

• 出版日期2016-2