Development of hybrid human interferon alfa-2 strain-producers and the use of enteropeptidase for production of N-terminal methionine-free interferons

作者：Shirokov D A^*; Ryabichenko V V; Akishina R I; Ospelnikova T P; Glazunov A V; Chestukhina G G; Veiko V P

来源：Molecular Biology, 2011, 45(3): 466-471.

DOI：10.1134/S0026893311020178

• interferon-alpha 2a
• interferon-alpha 2b
• enteropeptidase
• enterokinase

We developed a technique for production of human interferon-alpha 2a (IFN-alpha 2a) and IFN-alpha 2b lacking the N-terminal methionine. First of all, we constructed plasmids containing genes of hybrid IFN-alpha 2 controlled by different promoters; the proximal part of the genes incorporated various sequences encoding the enteropeptidase cleavage site. As a result, four strains of Escherichia coli producing hybrid IFN-alpha 2 were obtained. The protocol for IFN-alpha 2 renaturation, cleavege of its N-terminal part, and chromatographic purification of the N-terminal methionine-free IFN-alpha 2 was developed.

• 出版日期2011-6