Morphogenesis of respiratory syncytial virus in human primary nasal ciliated epithelial cells occurs at surface membrane microdomains that are distinct from cilia

作者：Jumat Muhammad Raihan; Yan Yan; Ravi Laxmi Iyer; Wong Puisan; Tra Nguyen Huong; Li Chunwei; Boon Huan Tan; De Yun Wang; Sugrue Richard J^*

来源：Virology, 2015, 484: 395-411.

DOI：10.1016/j.virol.2015.05.014

• Respiratory syncytial virus
• Virus morphogenesis
• Ciliated epithelial cells
• Human nasal epithelial stem/progenitor cells
• F-actin

The distribution of cilia and the respiratory syncytial virus (RSV) nucleocapsid (N) protein, fusion (F) protein, attachment (G) protein, and M2-1 protein in human ciliated nasal epithelial cells was examined at between 1 and 5 days post-infection (dpi). All virus structural proteins were localized at cell surface projections that were distinct from cilia. The F protein was also trafficked into the cilia, and while its presence increased as the infection proceeded, the N protein was not detected in the cilia at any time of infection. The presence of the F protein in the cilia correlated with cellular changes in the cilia and reduced cilia function. At 5 dpi extensive cilia loss and further reduced cilia function was noted. These data suggested that although RSV morphogenesis occurs at non-cilia locations on ciliated nasal epithelial cells, RSV infection induces changes in the cilia body that leads to extensive cilia loss.

• 出版日期2015-10
• 单位南阳理工学院