A preferable approach to clone hLIF cDNA from the genomic DNA

作者：Cui, Yihong^*; Zhu, Guangqin; Chen, Qiuju; Wang, Yunfei; Yang, Mingming; Song, Yuxuan; Wang, Jiangang; Cao, Binyun

来源：Electronic Journal of Biotechnology, 2011, 14(3): 10.

DOI：10.2225/vol14-issue3-fulltext-10

• cDNA cloning
• hLIF
• oral mucous membrane
• overlap PCR

Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired within a few hours by means of amplification of each exon and splicing using overlap-PCR. Thus, the new approach developed in this study is simple, time-and cost-effective, and it is not limited to particular gene expression levels of each tissue.

• 出版日期2011-5-15
• 单位西北农林科技大学