Detection of Anaplasma platys in dogs using real-time loop-mediated. isothermal amplification

作者：Li, Hua-tao; Sun, Ling-suang; Chen, Zhong-ming; Hu, Jing-si; Ye, Cun-dong; Jia, Kun; Wang, Heng; Yuan, Li-guo; Zhang, Gui-hong; Li, Shoujun^*

来源：Veterinary Journal, 2014, 199(3): 468-470.

DOI：10.1016/j.tvjl.2013.11.012

• Canine
• Anaplasma platys
• gltA gene
• Real-time loop-mediated isothermal amplification

Anaplasma platys is a parasite of canine platelets that causes infectious cyclic thrombocytopenia. In this study, a novel real-time loop-mediated isothermal amplification (RT-LAMP) method was developed to detect A. platys. RT-LAMP primer sets were designed using a citrate synthase gene sequence and the assay was performed at 63 degrees C for 30 mm. No cross-reactivity was observed with other Anaplasma or Ehrlichia spp. and the method exhibited a similar level of sensitivity in detecting the organism in 58 canine blood samples to that of a nested PCR. This RT-LAMP is a rapid and potentially cost-effective method of diagnosing A. platys infection in dogs.

• 出版日期2014-3
• 单位华南农业大学