A High Frequency of Peripheral Blood IL-22(+)CD4(+) T Cells in Patients With New Onset Type 2 Diabetes Mellitus

作者：Guo, Hui; Xu, Bing-Chuan; Yang, Xi-Ge; Peng, Di; Wang, Ye; Liu, Xiao-Bo; Cui, Cheng-Ri; Jiang, Yan-Fang^*

来源：Journal of Clinical Laboratory Analysis, 2016, 30(2): 95-102.

DOI：10.1002/jcla.21821

• type 2 diabetes mellitus
• Th22
• Th17

BackgroundThis study is aimed at investigating the frequency of different functional IL-22(+)CD4(+) T cells in Chinese patients with type 2 diabetes mellitus (T2DM).
MethodsThe frequency of circulating IFN-+IL-17-IL-22-CD4(+) (Th1), IFN--IL-17A+IL-22-CD4(+) (Th17), and IFN--IL-17A-IL-22(+)CD4(+) (Th22), and other subsets of IL-22(+)CD4(+) T cells in 31 patients with new onset T2DM and 16 healthy controls was characterized by flow cytometry. The levels of serum IL-22, IL-17, IFN-, insulin C-peptide, hemoglobin A1c (HbA1c), fasting plasma glucose, and insulin were examined.
ResultsThe frequency of Th1, Th17, Th22, IFN-+IL-17(-)IL-22(+), and IFN--IL-17(+)IL-22(+) CD4(+) T cells and the concentrations of IL-22, but not IL-17 and IFN, in the patients were significantly higher than controls. The percentages of Th22 cells were correlated positively with the frequency of IFN--IL-17(+)IL-22(+)CD4(+) T cells, the values of body mass index (BMI) and homeostatic model assessment insulin resistance (HOMA-IR), and the levels of serum IL-22 in those patients.
ConclusionOur data suggest that IL-22(+)CD4(+) T cells may contribute to the early process of T2DM.

• 出版日期2016-3
• 单位吉林大学