Nano-engineered Flavin-Dependent Glucose Dehydrogenase/Gold Nanoparticle-Modified Electrodes for Glucose Sensing and Biofuel Cell Applications

作者：Yehezkeli Omer; Tel Vered Ran; Reichlin Sara; Willner Itamer^*

来源：ACS Nano, 2011, 5(3): 2385-2391.

DOI：10.1021/nn200313t

• biosensor
• biofuel cell
• glucose
• Au nanoparticles
• glucose dehydrogenase
• electropolymerization

A three-dimensional composite consisting of the oxygen-insensitive flavin-dependent glucose dehydrogenase, GDH, and Au nanoparticles (NPs) is assembled on a Au surface using an electropolymerization process: The bls-aniline-cross-linked GDH/Au NPs composite reveals effective electrical contact with the electrode (k(et) =1100s(1)(-1)), and the effective bioelectrocatalyzed oxidation Is driven by the enzyme/NPs matrix. The GDH/Au NPs-functionalized electrode Is implemented as an amperometric glucose sensor,. and it superior functions when compared to an analogous glucose oxidase/Au NPs system. The O-2-insensitive GDH/Au NPs composite electrode was further used as an anode In a membraneless glucose/O-2 biofuel cell. The cathode in this system was composed of bilirubin oxidase cross linked onto a carbon nanotube-modified glassy carbon electrode. The power output of the cell was 32 mu W cm(-2)

• 出版日期2011-3