A Protein Chip Based Inhibitor Screening for Influenza Neuraminidases: the Importance of Glycan-specific Recognition

作者：Lee Jung kyu; Park Chan Won; Kwon Hyuk Ku; Jung Seunho; Jeong Hyun Ja; Kang In Cheol; Choi Youngjin^*

来源：BioChip Journal, 2014, 8(3): 209-217.

DOI：10.1007/s13206-014-8307-8

• Carbohydrate-protein interaction
• Drug screening
• Neuraminidase
• Protein chip
• Anti-viral

A protein chip assay system based on a specific carbohydrate-protein interaction has been constructed for the identification of chemical inhibitors of influenza neuraminidase. The devised chip is composed of three layered proteins, that is, a matrix, a probe, and a neuraminidase protein. Transferrin, the matrix protein, was immobilized onto a chip panel to provide biological glycan chains. Fluorescence-labeled lectin or hemagglutinin was added as a glycan-interacting protein probe. Finally specific sialic acid moiety on the transferrin was then cleaved by an avian or human influenza neuraminidase. Each lectin or hemagglutinin showed different glycan specificity as a result of catalytic action of different influenza neuraminidase. Using this principle, we finally, tested the neuraminidase of strain H1N1 as a model system to verify the screening capability of this protein chip approach.

• 出版日期2014-9-20