Aldehyde dehydrogenase activity identifies a subpopulation of canine adipose-derived stem cells with higher differentiation potential

作者：Itoh Harumichi; Nishikawa Shimpei^*; Haraguchi Tomoya; Arikawa Yu; Hiyama Masato; Eto Shotaro; Iseri Toshie; Itoh Yoshiki; Tani Kenji; Nakaichi Munekazu; Taura Yasuho; Itamoto Kazuhito^*

来源：Journal of Veterinary Medical Science, 2017, 79(9): 1540-1544.

DOI：10.1292/jvms.16-0503

• adipose-derived stem cells
• aldehyde dehydrogenase activity
• flow cytometry
• osteogenic differentiation
• adipogenic differentiation

Adipose-derived stem cells (ADSCs) are abundant and readily obtained, and have been studied for their clinical applicability in regenerative medicine. Some surface antigens have been identified as markers of different ADSC subpopulations in mice and humans. However, it is unclear whether functionally distinct subpopulations exist in dogs. To address this issue, we evaluated aldehyde dehydrogenase (ALDH) activity-a widely used stem cell marker in mice and humans-by flow cytometry. Approximately 20% of bulk ADSCs showed high ALDH activity. Compared to cells with low activity (ALDHLo), the high-activity (ALDHHi) subpopulation exhibited a higher capacity for adipogenic and osteogenic differentiation. This is the first report of distinct ADSC subpopulations in dogs that differ in terms of adipogenic and osteogenic differentiation potential.

• 出版日期2017-9