科研之友
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摘要
SPLITT Fractionation (SF) provides separation of sample into two subpopulations. Separation into more than two subpopulations requires repeated SF operations. In this study, two Gravitation SF (GSF) channels were connected in a series (Tandem GSF) to obtain a separation into three subpopulations and to improve the fractionation efficiency (FE) of the fraction-b in the full-feed depletion (FFD) mode. In a single channel FFD-GSF operation, the fraction-a contained mostly the beads smaller than the cutoff diameter (d(c)), while the fraction-b contained beads smaller than dc as well as those larger than d(c), as expected. The measured FEs of the fraction-b are much lower than those of the fraction-a in all cases. The FE's of the fraction-a are higher than 84% with the average of about 91%, while those of the fraction-b are lower than 60% with the average of about 43%. No particular trends were found between FE and d(c), indicating the performance of FFD-GSF does not change with de in the range where tested. Also no clear trends were observed between the FE and the sample-feeding flow rate, indicating higher sample-feeding rate can be used to increase the sample throughput without losing resolution. When two GSF channels were connected so that the flow stream emerging from the outlet-b of the channel-1 is fed directly into the channel-2, all three FEs measured for the fraction-la were high with the average value of 99%, indicating it contains almost purely the beads smaller than de. The FEs measured for the fraction-2a are still good with the average value of 92%. The FEs measured for the fraction-2b are 64% in average, which is about 20% improvement from those obtained in a single channel FFD-GSF at the same conditions.
- 出版日期2011-2-20
