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摘要
An immunomagnetic-bead-based enzyme-linked immunosorbent assay (IMB-ELISA) was developed for detection of pesticides by using carboxyl functionalized magnetic Fe3O4 nanoparticles (CMNPs). The CMNPs were prepared by co-precipitation of Fe2+/Fe3+ with oleic acid as a surfactant and subsequent oxidation of C=C into COOH by KMnO4 solution in situ. Then, anti-pesticide (triazophos) monoclonal antibodies were directly bonded onto the magnetic nanoparticles, which significantly increased the sensitivity compared with classic ELISA. The detection limit was 0.10 ng mL(-1). Addition-recovery and high-precision experiments were performed on blank samples that were determined to be without triazophos. The average recovery rate for three types of samples (with each spiking concentration measured 5 times in parallel) ranged from 83.1% to 115.9%, with a relative standard deviation (RSD) of less than 10%, which meets the requirement of pesticide residue analysis. The application results were in accordance with the gas chromatography-mass spectrometry (GC-MS) method, suggesting that IMB-ELISA is rapid and reliable for pesticide detection.
- 出版日期2015
- 单位中国农业科学院农业质量标准与检测技术研究所
