摘要
In this study, the effects of indigenous probiotic, Lactobacillus rhamnosus, as an additive to water in Portunus pelagicus larviculture was investigated on survival rate, digestive enzyme activities and water quality. All experiments were triplicated and designed with three different administrations of probiotic 1.0 x 10(6) cfu mL(-1) (T-1), 5.0 x 10(6) cfu mL(-1) (T-2) and 1.0 x 107 cfu mL(-1) (T-3) added daily and one control without any probiotic. The supplementation of the probiotic significantly (p < 0.05) increased survival rate for all treatments over the controls. Highest survival rate (10.0 +/- 0.9%) of larvae was achieved in T-2. Addition of probiotic bacteria affected the bacterial load (cfu mL(-1)) in rearing water. The specific activities of protease and amylase enzymes in probiotic treatments were significantly higher (p < 0.05). The highest specific activities of protease (0.17 +/- 0.01 U mg(-1) protein) and amylase (0.36 +/- 0.02 U mg(-1) protein) were determined in treated group T-2, respectively. Addition of probiotic affected the pH and nitrogen content of rearing water. The results of this study suggest that the indigenous L. rhamnosus added to rearing water at a certain concentration significantly enhanced survival rate, measured digestive enzyme activities and improved the water quality of P. pelagicus larvae.
- 出版日期2013-9