NMR Characterization of the Interaction of the Salmonella Type III Secretion System Protein SipD and Bile Salts

作者：Wang Yu; Nordhues Bryce A; Zhong Dalian; De Guzman Roberto N^*

来源：Biochemistry, 2010, 49(19): 4220-4226.

DOI：10.1021/bi100335u

Salmonella and Shigella bacteria require the type III secretion system (T3SS) to inject virulence proteins into their hosts and initiate infections. The tip proteins SipD and Ipa D are critical components of the Salmonella and Shigella T3SS, respectively. Recently, SipD and IpaD have been shown to interact with bile salts, which are enriched in the intestines, and are hypothesized to act as environmental sensors for these enteric pathogens. Bile salts activate the Shigella T3SS but repress the Salmonella T3SS, and the mechanism of this differing response to bile salts is poorly understood. Further, how SipD binds to bile salts is currently unknown. Computer modeling predicted that Ipa D binds the bile salt deoxycholate in a cleft formed by the N-terminal domain and the long central coiled coil of IpaD. Here, we used NMR methods to determine which SipD residues are affected by the interaction with the bile salts deoxycholate, chenodeoxycholate, and taurodeoxcholate. The bile salts perturbed nearly the same set of SipD residues: however, the largest chemical shift perturbations occurred away from what was predicted for the bile salt binding site in Ipa D. Our NMR results indicate that that bile salt interaction of SipD will be different from what was predicted for IpaD, suggesting a possible mechanism for the differing response of Salmonella and Shigella to bile salts.

• 出版日期2010-5-18