An Enzyme-entrapped Agarose Gel for Visualization of Ischemia-induced L-Glutamate Fluxes in Hippocampal Slices in a Flow System

作者：Tanaka Kazuhisa; Shoji Atushi; Sugawara Masao^*

来源：Analytical Sciences, 2015, 31(4): 321-325.

DOI：10.2116/analsci.31.321

• Enzyme-entrapped agarose gel
• imaging of L-glutamate
• hippocampal slice
• ischemia

An agarose gel slip containing L-glutamate oxidase (GluOx), horseradish peroxidase (HRP) and a dye DA-64 is proposed as a tool for visualizing ischemia-induced L-glutamate release in hippocampal slices in a flow system. The agarose slip with a detection limit of 6.0 +/- 0.8 mu mol L-1 for L-glutamate enabled us to visualize L-glutamate fluxes in a flow system. The leak of a dye from the agarose gel was negligible and a diffusion blur due to spreading of Bindshedler's Green (BG) within the gel was suppressed. Monitoring the time-dependent change of ischemia-induced L-glutamate fluxes at neuronal regions CA1, DG and CA3 of hippocampal slices is demonstrated.

• 出版日期2015-4