PhyloToAST: Bioinformatics tools for species-level analysis and visualization of complex microbial datasets

作者:Dabdoub Shareef M; Fellows Megan L; Paropkari Akshay D; Mason Matthew R; Huja Sarandeep S; Tsigarida Alexandra A; Kumar Purnima S*
来源:Scientific Reports, 2016, 6(1): 29123.
DOI:10.1038/srep29123

摘要

The 16S rRNA gene is widely used for taxonomic profiling of microbial ecosystems; and recent advances in sequencing chemistry have allowed extremely large numbers of sequences to be generated from minimal amounts of biological samples. Analysis speed and resolution of data to species-level taxa are two important factors in large-scale explorations of complex microbiomes using 16S sequencing. We present here new software, Phylogenetic Tools for Analysis of Species-level Taxa (PhyloToAST), that completely integrates with the QIIME pipeline to improve analysis speed, reduce primer bias (requiring two sequencing primers), enhance species-level analysis, and add new visualization tools. The code is free and open source, and can be accessed at http://phylotoast.org.

  • 出版日期2016-6-30