Microdialysis sampling coupled to on-line high-performance liquid chromatography for determination of arbutin in whitening cosmetics

作者：Lin CH; Wu HL; Huang YL^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2005, 829(1-2): 149-152.

DOI：10.1016/j.jchromb.2005.10.008

• microdialysis sampling
• high-performance liquid chromatography
• arbutin
• whitening cosmetics
• validation

We have developed a method that uses on-line microdialysis sampling coupled with high-performance liquid chromatography (HPLC) to determine arbutin in whitening cosmetics. The optimum analytical conditions for microdialysis sampling were a probe length of 10 mm and a dialysis flow-rate of 5 mu l min(-1). The accuracy (% bias) for intra-day (n = 6) and inter-day (it = 30, five consecutive days) analyses ranged from -8.9 to 11.5%, with a precision below 7.64% R.S.D. The calibration curve was linear within the range from 0.1 to 20 mM (R-2 = 0.9989). The detection limit was 15 mu M. By comparing the arbutin levels determined this way in the whitening cosmetics with the results obtained from the no-net-flux method, we conclude that our proposed on-line microdialysis-HPLC system displays good accuracy. We evaluated the robustness of our optimum conditions by means of a Plackett-Burman design. Apart from the effect of a low flow-rate of perfusate-an increase of 12.52 +/- 2.31%-we observed no significant changes in the analyses upon changing the levels of any other parameter. Because this on-line method offers the advantages of simplicity, reliability, the lack of any tedious sample pretreatment process, and a reduced use of organic solvents, we believe that it is suitable for the routine analysis of commercial cosmetics.

• 出版日期2005-12-27