An effective ICT-based and ratiometric fluorescent probe for sensing sulfite

作者：Zhang, Li-Jie; Wang, Zhao-Yang; Cao, Xiang-Jian; Liu, Jin-Ting^*; Zhao, Bao-Xiang^*

来源：Sensors and Actuators B: Chemical , 2016, 236: 741-748.

DOI：10.1016/j.snb.2016.06.055

• Fluorescent probe
• Ratiometric
• Sulfite
• Cell image
• Theoretical calculation
• Exogenous
• Endogenous

We have developed a ratiometric fluorescent probe based on ICT mechanism for imaging intracellular sulfite (SO32) in HeLa cells. The probe could selectively stain cells with scarce cytotoxicity and excellent photostability. The probe displayed a high selectivity for sulfite over other anions and active small biological molecules including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) at room temperature. The reaction could complete within 30 s and detection limit was as low as 76 nM, which is superior to some reported probes. The detecting mechanism was proved by MS, H-1 NMR and theoretical calculation. The probe can also detect endogenous sulfite in HepG2 cells.

• 出版日期2016-11-29
• 单位山东大学