Detection of gamma-tubulin in spermatogonial cells of Bombyx mori (Lepidoptera) and Chortophaga viridifasciata (Orthoptera)

作者：Matsuda Yumi; Sahara Ken; Yasukochi Yuji; Yamashiki Naoko^*

来源：Zoological Science, 2007, 24(8): 781-786.

DOI：10.2108/zsj.24.781

• BAC-FISH
• centrosome
• gamma-tubulin
• grasshopper
• silkworm

We detected a putative gamma-tubulin gene in sifico and detected BACs containing the gene from a Bombyx mori BAC library. BAC-FISH mapping revealed that the gene is located on chromosome 5. To observe the distribution of gamma-tubulin, we employed antibodies against mammalian gamma-tubulin peptides. Western blot analysis disclosed a band very similar in size to gamma-tubulin protein in other species (approximately 48 kDa). In mitotic metaphase of B. mori spermatogonial cells, gamma-tubulin is exclusively localized in the spindle poles, where the centrosomes occur. We applied the same system to the grasshopper Chortophaga viridifasciata, as a representative of insect orders in which the gamma-tubulin distribution had not previously been studied. gamma-Tubulin was also found in the spindle poles during metaphase of spermatogonial cells in the grasshopper.

• 出版日期2007-8