Alpha-mangostin suppresses IL-6 and IL-8 expression in P. gingivalis LPS-stimulated human gingival fibroblasts

作者：Yiemwattana Ichaya^*; Kaomongkolgit Ruchada****

来源：Odontology, 2015, 103(3): 348-355.

DOI：10.1007/s10266-014-0160-7

• Alpha-mangostin
• HGFs
• IL-6/8

This study aims to investigate the effect of alpha-mangostin on interleukin (IL)-6 and IL-8 expression in human gingival fibroblasts (HGFs). HGFs were challenged with Porphyromonas gingivalis LPS and then treated with various concentrations of alpha-mangostin. The cytotoxicity was determined using MTS assay and cytokine expressions were evaluated by Real-time PCR and ELISA. The results showed that 5 mu g/ml P. gingivalis LPS and alpha-mangostin at 1 mu g/ml or less did not affect the viability of HGFs. Alpha-mangostin reduced IL-6 and IL-8 mRNA and protein in P. gingivalis LPS-stimulated HGFs. These findings suggested that alpha-mangostin might be used as an adjunct to the periodontal therapy.

• 出版日期2015-9