Application of Reproductive Biotechnology for the Recovery of Endangered Breeds: Birth of the First Calf of Murciana-Levantina Bovine Breed Derived by OPU, In vitro Production and Embryo Vitrification

作者：Ruiz S^*; Romero Aguirregomezcorta J; Astiz S; Peinado B; Almela L; Poto A

来源：Reproduction in Domestic Animals, 2013, 48(6): e81-e84.

DOI：10.1111/rda.12179

Contents In a conservation project, reproductive biotechnology was implemented for the recovery and conservation of an endangered bovine breed in Spain. The breed Murciana-Levantina, declared to be worthy of special protection status (http://www.fao.org/newsroom/en/news/2006/1000464/index.html), is of great interest because of its hardness, longevity, docility and disease resistance. This contribution describes the birth of the first calf of this breed obtained by reproductive biotechnology, using ultrasound-guided punction and aspiration of ovarian follicles, in vitro embryo production, vitrification of embryos by a cryotop device and, finally, the transfer of cryopreserved embryos to recipient heifers of a commercial dairy herd.

• 出版日期2013-12