A label-free kissing complexes-induced fluorescence aptasensor using DNA-templated silver nanoclusters as a signal transducer

作者：Zhang Kai^*; Wang Ke; Zhu Xue; Xie Minhao

来源：Biosensors and Bioelectronics, 2016, 78: 154-159.

DOI：10.1016/j.bios.2015.11.038

• Riboswitches
• Kissing complexes-induced aptasensor
• Adenosine
• Adenosine deaminase

Riboswitches are complex folded RNA domains that serve as receptors for specific metabolites which identified in prokaryotes. They are comprised of a biosensor that includes the binding site for a small ligand and they respond to association with this ligand by undergoing a conformational change. In the present study, we report on the integration of silver nanoclusters (AgNCs) and riboswitches for the development of a kissing complexes-induced aptasensor (KCIA). We specifically apply the tunable riboswitches properties of this strategy to demonstrate the multiplexes analysis of adenosine and adenosine deaminase (ADA). This strategy allows for simple tethering of the specific oligonucleotides stabilizing the AgNCs to the nucleic acid probes. This is a new concept for aptasensors, and opens an opportunity for design of more novel biosensors based on the kissing complexes-induced strategy.

• 出版日期2016-4-15
• 单位江苏省原子医学研究所