Difference in Uptake of Tetrodotoxin and Saxitoxins into Liver Tissue Slices among Pufferfish, Boxfish and Porcupinefish

作者：Nagashima Yuji^*; Ohta Akira; Yin Xianzhe; Ishizaki Shoichiro; Matsumoto Takuya; Doi Hiroyuki; Ishibashi Toshiaki

来源：Marine Drugs, 2018, 16(1): 17.

DOI：10.3390/md16010017

• pufferfish
• boxfish
• porcupinefish
• tetrodotoxin
• saxitoxins
• liver tissue slice
• in vitro incubation
• paralytic shellfish toxins

Although pufferfish of the family Tetraodontidae contain high levels of tetrodotoxin (TTX) mainly in the liver, some species of pufferfish, boxfish of the family Ostraciidae, and porcupinefish of the family Diodontidae do not. To clarify the mechanisms, uptake of TTX and saxitoxins (STXs) into liver tissue slices of pufferfish, boxfish and porcupinefish was examined. Liver tissue slices of the pufferfish (toxic species Takifugu rubripes and non-toxic species Lagocephalus spadiceus, L. cheesemanii and Sphoeroides pachygaster) incubated with 50 mu M TTX accumulated TTX (0.99-1.55 mu g TTX/mg protein) after 8 h, regardless of the toxicity of the species. In contrast, in liver tissue slices of boxfish (Ostracion immaculatus) and porcupinefish (Diodon holocanthus, D. liturosus, D. hystrix and Chilomycterus reticulatus), TTX content did not increase with incubation time, and was about 0.1 mu g TTX/mg protein. When liver tissue slices were incubated with 50 mu M STXs for 8 h, the STXs content was <0.1 mu g STXs/mg protein, irrespective of the fish species. These findings indicate that, like the toxic species of pufferfish T. rubripes, non-toxic species such as L. spadiceus, L. cheesemanii and S. pachygaster, potentially take up TTX into the liver, while non-toxic boxfish and porcupinefish do not take up either TTX or STXs.

• 出版日期2018-1