Antiparasitic efficacy of Gracillin and Zingibernsis newsaponin from Costus speciosus (Koen ex. Retz) Sm. against Ichthyophthirius multifiliis

作者：Zheng, Wei^*; Yan, Chun-Mei; Zhang, Ya-Bin; Li, Ze-Hong; Li, Zhongqiang; Li, Xiu-Ying; Wang, Zhang-Wei; Wang, Xiulan; Chen, Wei-Qiang; Yu, Xin-Hai

来源：Parasitology, 2015, 142(3): 473-479.

DOI：10.1017/S0031182014001358

• Ichthyophthirius multifiliis
• Gracillin
• Zingibernsis newsaponin
• antiparasitic

The present study aims to evaluate the antiparasitic activity of active components from Costus speciosus against Ichthyophthirius multifiliis. Bioassay-guided fractionation was employed to identify active compounds from C. speciosus yielding 2 bioactive compounds: Gracillin and Zingibernsis newsaponin. In-vitro assays revealed that Gracillin and Zingibernsis newsaponin could be 100% effective against I. multifiliis at concentrations of 08 and 45mgL(-1), with median effective concentration (EC50) values of 053 and 32mgL(-1), respectively. All protomonts and encysted tomonts were killed when the concentrations of Gracillin and Zingibernsis newsaponin were 10 and 50mgL(-1). In-vivo experiments demonstrated that fish treated with Gracillin and Zingibernsis newsaponin at concentrations of 10 and 50mgL(-1) carried significantly fewer parasites than the control (P<005). Mortality of fish did not occur in the treatment group (Zingibernsis newsaponin at 50mgL(-1)) during the trial, although 100% of untreated fish died. Acute toxicities (LD50) of Gracillin and Zingibernsis newsaponin for grass carp were 164 and 207mgL(-1), respectively. These results provided evidence that the 2 compounds can be selected as lead compounds for the development of new drugs against I. multifiliis.

• 出版日期2015-3
• 单位吉林农业大学