α-Lipoic acid ameliorates impaired glucose uptake in LYRM1 overexpressing 3T3-L1 adipocytes through the IRS-1/Akt signaling pathway

作者：Qin, Zhen-Ying; Zhang, Min; Guo, Xi-Rong; Wang, Yu-Mei; Zhu, Guan-Zhong; Ni, Yu-Hui; Zhao, Ya-Ping; Qiu, Jie; Kou, Chun-Zhao; Qin, Rui^*; Cao, Xin-Guo

来源：Journal of Bioenergetics and Biomembranes, 2012, 44(5): 579-586.

DOI：10.1007/s10863-012-9460-1

• alpha-LA
• LYRM1
• Mitochondrial dysfunction
• Insulin resistance
• Obesity

Overexpression of the Homo sapiens LYR motif containing 1 (LYRM1) causes mitochondrial dysfunction and induces insulin resistance in 3T3-L1 adipocytes. alpha-Lipoic acid (alpha-LA), a dithiol compound with antioxidant properties, improves glucose transport and utilization in 3T3-L1 adipocytes. The aim of this study was to investigate the direct effects of alpha-LA on reactive oxygen species (ROS) production and insulin sensitivity in LYRM1 overexpressing 3T3-L1 adipocytes and to explore the underlying mechanism. Pretreatment with alpha-LA significantly increased both basal and insulin-stimulated glucose uptake and insulin-stimulated GLUT4 translocation, while intracellular ROS levels in LYRM1 overexpressing 3T3-L1 adipocytes were decreased. These changes were accompanied by a marked upregulation in expression of insulin-stimulated tyrosine phosphorylation of IRS-1 and serine phosphorylation of Akt following treatment with alpha-LA. These results indicated that alpha-LA protects 3T3-L1 adipocytes from LYRM1-induced insulin resistance partially via its capacity to restore mitochondrial function and/or increase phosphorylation of IRS-1 and Akt.

• 出版日期2012-10
• 单位南京医科大学