科研之友
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摘要
Lysophosphatidic acid (LPA) is a lipid growth factor that exerts diverse biological effects through its cognate receptors (LPA(1)-LPA(6)). LPA(1), which is predominantly expressed in the brain, plays a pivotal role in brain development. However, the role of LPA(1) in neuronal migration has not yet been fully elucidated. Here, we delivered LPA(1) to mouse cerebral cortex using in utero electroporation. We demonstrated that neuronal migration in the cerebral cortex was not affected by the overexpression of LPA(1). Moreover, these results can be applied to the identification of the localization of LPA(1). The subcellular localization of LPA(1) was endogenously present in the perinuclear area, and overexpressed LPA(1) was located in the plasma membrane. Furthermore, LPA(1) in developing mouse cerebral cortex was mainly expressed in the ventricular zone and the cortical plate. In summary, the overexpression of LPA(1) did not affect neuronal migration, and the protein expression of LPA(1) was mainly located in the ventricular zone and cortical plate within the developing mouse cerebral cortex. These studies have provided information on the role of LPA(1) in brain development and on the technical advantages of in utero electroporation.
- 出版日期2014-7-31
