Macrophage-dependent impairment of alpha(2)-adrenergic autoreceptor inhibition of Ca2+ channels in sympathetic neurons from DOCA-salt but not high-fat diet-induced hypertensive rats

作者：Mui Ryan K; Fernandes Roxanne N; Garver Hannah G; Van Rooijen Nico; Galligan James J^*

来源：American Journal of Physiology - Heart and Circulatory Physiology, 2018, 314(4): H863-H877.

DOI：10.1152/ajpheart.00536.2017

• alpha(2)-adrenergic system
• macrophages and reactive oxygen species
• obesity-associated hypertension
• salt-sensitive hypertension
• sympathetic nervous system

DOCA-salt and obesity-related hypertension are associated with inflammation and sympathetic nervous system hyperactivity. Prejunctional alpha(2)-adrenergic receptors (alpha(2)ARs) provide negative feedback to norepinephrine release from sympathetic nerves through inhibition of N-type Ca2+ channels. Increased neuronal norepinephrine release in DOCA-salt and obesity-related hypertension occurs through impaired alpha(2)AR signaling; however, the mechanisms involved are unclear. Mesenteric arteries are resistance arteries that receive sympathetic innervation from the superior mesenteric and celiac ganglia (SMCG). We tested the hypothesis that macrophages impair alpha(2)AR-mediated inhibition of Ca2+ channels in SMCG neurons from DOCA-salt and high-fat diet (HFD)-induced hypertensive rats. Whole cell patch-clamp methods were used to record Ca2+ currents from SMCG neurons maintained in primary culture. We found that DOCA-salt, but not HFD-induced, hypertension caused macrophage accumulation in mesenteric arteries, increased SMCG mRNA levels of monocyte chemoattractant protein-1 and tumor necrosis factor-alpha, and impaired alpha(2)AR-mediated inhibition of Ca2+ currents in SMCG neurons. alpha(2)AR dysfunction did not involve changes in alpha(2)AR expression, desensitization, or downstream signaling factors. Oxidative stress impaired alpha(2)AR-mediated inhibition of Ca2+ currents in SMCG neurons and resulted in receptor internalization in human embryonic kidney-293T cells. Systemic clodronate-induced macrophage depletion preserved alpha(2)AR function and lowered blood pressure in DOCA-salt rats. HFD caused hypertension without obesity in Sprague-Dawley rats and hypertension with obesity in Dahl salt-sensitive rats. HFD-induced hypertension was not associated with inflammation in SMCG and mesenteric arteries or alpha(2)AR dysfunction in SMCG neurons. These results suggest that macrophage-mediated alpha(2)AR dysfunction in the mesenteric circulation may only be relevant to mineralocorticoid-salt excess.
NEW & NOTEWORTHY Here, we identify a contribution of macrophages to hypertension development through impaired alpha(2)-adrenergic receptor (alpha(2)AR)-mediated inhibition of sympathetic nerve terminal Ca2+ channels in DOCA-salt hypertensive rats. Impaired alpha(2)AR function may involve oxidative stress-induced receptor internalization. alpha(2)AR dysfunction may be unique to mineralocorticoid-salt excess, as it does not occur in obesity-related hypertension.

• 出版日期2018-4